The 2001 MTV VMAs-An Overview essays

The 2001 MTV VMAs-An Overview articles 2001 has been an extraordinary year for the music and diversion channel, MTV. New periods of The Real Word and Road Rules, different shows and specials, and the Movie Awards have all been huge features of this current year up until now. The channel likewise praised its twentieth Anniversary this past August. Be that as it may, seemingly, the greatest occasion of them everything is the Video Music Awards. Known for its propensity to stretch the limits with regards to what can and cant be appeared on satellite TV, the VMAs are more about the show than the honors. This year was no exemption. Live from the Metropolitan Opera House, the show opened with have Jamie Foxx playing out an exceptionally fitting drama interpretation of a few of this previous years most famous melodies. At that point, he immediately changed gears into a monolog, offending a few big names that were available at the honors, as opposed to prior reports that he wouldnt do as such. Fortunately, Will Ferrell of Saturday Night Live notoriety ran onto the phase in Foxxs act, ascending a prop in front of an audience while yelling, Im seething! This was a reverence to a comparable demonstration performed by the bassist of the band Rage Against the Machine from a years ago VMAs, and constrained MTV to slice to a business break. In this manner started a consistent stream of exhibitions and grants. A fascinating subject to the VMAs this year was the wilderness. Before the chosen people for each grant were named, little nature narrative clasps were appeared, in any case, in evident MTV design, a large portion of them were somewhat provocative. Britney Spears lip-synchronizing execution likewise highlighted a zoo-loaded with confined creatures behind her, also a snake around her neck. Another snapshot of the VMAs that can't be neglected is the tribute to Aaliyah. A few specialists including Janet Jackson and Missy Elliot said a couple of words. Aaliyahs sibling additionally delivered a somewhat moving discourse. The second wouldve been great, had Janet Jackson not read t ... <!

Ethics in Retail Industry free essay sample

The investigation additionally includes how associations guarantee best moral practices and whether a socially dependable retailer increases upper hand and productivity through its moral practices. 25 December 2012 00:27 Methodology: Studying on previously mentioned issues can be begun from prevailing upon how morals and social duty affected the hierarchical procedures as of late. A short investigation of the retail associations, known as ‘ethical’, uncovers how the associations are pressurized to make such morally maintainable picture for its clients. Examining different models, the variables, impacting the moral acts of an association, can be identified with discover the stuff to be characterized as a socially dependable retailer. At long last the between relationship of gainfulness and moral practices can be set up to close the exhibition of retailing industry as far as morals and social duty. The methodologies on moral acts of certain association can be gotten to from the cases concentrates on these retail enterprises. The powerful factors can be recognized from the regular patterns in these methodologies and a decisive story through client observations can be drawn by direct customer contact and individual meetings. As a foundation of any association and individual while cooperating with different partners, inside a retailing industry certain measures are followed to manage the dynamic minutes. The extent of the article incorporates investigation of these practices according to moral perspective, taking models from goliath retail chains and furthermore investigation of moral view of the business power in retailing industry. The examination additionally incorporates how associations guarantee best moral practices and whether a socially dependable retailer increases upper hand and productivity through its moral practices. *Methodology:* [Quoted content hidden] 2 of 2 31/01/2013 10:37 PM Executive Summary: As a foundation of any association and individual while communicating with different partners, inside a retailing industry certain principles are followed to manage the dynamic minutes. This article incorporates investigation of these practices according to moral perspective, taking models from mammoth retail chains and furthermore investigation of moral impression of the business power in retailing industry. The investigation additionally includes how associations guarantee best moral practices and whether a socially mindful retailer increases upper hand and productivity through its moral practices. Different issues that may emerge from morally grieved circumstance where retail sales reps may wind up in moral predicament are talked about in various casing of references. Regularly normal untrustworthy practices in the retail business, for example, abuse of laborers, overemphasis on beneficial items, dishonestly fixing costs on items, exploitative issues of limited time crusade and their negative consequences for buyer mentality are talked about in detail. Subsequently untrustworthy practices in green retailing, the most recent buzz of the retail business in India are likewise talked about with models from universal retail chains. Through significant investigations it has been discovered that for retailers there are two sorts of moral code by and by: express and verifiable. Unequivocal code of moral practices indicates composed standards that retailers follow to work under. Certain code is characterized as the arrangement of unwritten however surely knew rules/norms of good obligations. In retailing condition Sales individuals interface with people from outside the store, clients and just as individuals from inside their association. The people with whom they cooperate may have certain dissimilar needs and issues that sales reps may fulfill to determine. Since these people need the sales reps to help cultivate their own need, fulfillment or issue, the requests conveyed to the salesmen by them are probably going to be different and regularly inconsistent. Therefore retail deals work force regularly face moral issues when conflicted between short run pressures from the executives I. e. to accomplish deals share and since quite a while ago run objectives of accomplishing client certainty and altruism. Furthermore the condition that the retail faculty works in is favorable for the improvement of moral issues due to the assortment of assignment they performs 3 hich can put them in awkward circumstances that may be conveniently tended to utilizing faulty conduct I. e. taking care of client return or trade. Further examinations have demonstrated that ordinarily retail deals work force get minimal formalized deals preparing and subsequently they have not been successfully taught about how the organization might want them to act in these mora lly upsetting circumstances. Administrators in retail industry ought to be worried about these circumstances looked by retail salesmen as failure to deal with such circumstance brings about bringing down occupation execution, disappointing client and so forth. Kinds of Ethical Problems in Retail Sector: There are various sorts of circumstances where moral issues can be happened in retail industry. From the differing individuals that retail sales reps connect including clients, friend and the executives anybody can imperil the moral standards of the circumstance. Utilizing these three gatherings of individuals as edge of reference moral issues can be sorted in three expansive territories; client, companions and business related circumstances. Client related circumstances may incorporate those circumstances that involve association with client and have direct effect upon clients. For instance neglecting to help clients or deliberately giving them off base change. Friend related circumstances include peer from non-work gatherings and individual representatives too. For instance offering or being compelled to give a companion or worker a markdown. Business related circumstances incorporate conditions that might be encouraged by organization working methodology, rehearses that are cultivated by workplace. For instance neglecting to acquire a check approval, selling an item as selective when it isn't could be remembered for exploitative practices. Basic untrustworthy practices in Retail Marketing.

Small Business Advise and Discussion Essay Example | Topics and Well Written Essays - 500 words

Private company Advise and Discussion - Essay Example These preparations incorporates the accompanying: 1. Legitimate Market Study: Analyzing the allure of the business, request and gracefully examination, client, contender investigation, SWOT examination of the organization, capital planning and money related getting ready for the business will help the entrepreneur to see all the little and enormous issues associated with the specific business. The objective market ought to be recognized and divided. The entrepreneurs face issues in raising the capital because of different reasons. These reasons can be chance, validity of the entrepreneur, advertise estimation of the tasks, anticipated development of the undertaking or the business, client base, business know-how of the proprietors, capabilities, experience and reinforcement to help any sort of monetary emergency. There are different elective wellsprings of account accessible for the little speculators. These are Boot Strap, Angel, Initial Public contribution and private placement.1 In the Boot Strap subsidizing the assets are raised with family, companions and others. On account of Angel the venture is finished by well off people. Private arrangements are done through banks and different ventures organizations. Organizations likewise access to the market through IPO to raise the capital. Boot Strap is a financing choice accessible for all sort of business. A large portion of the organizations fire up with the ventures this way. These sort of ventures which are finished with the assistance of different close circle individuals like family, companions, associates and at times great clients help entrepreneurs for their underlying phases of advancements. Entrepreneurs can successfully use these ventures. The outcomes delivered like benefit, expanded market esteem, client base and request of the items can pull in speculators for the further ventures. These can be new speculators, banks and other potential financial specialists. This can help the entrepreneurs to bring the assets up in the extension and development periods of the

The Forbidden Truth About The Road Mccarthy Essay Topics Exposed by an Old Pro

The Forbidden Truth About The Road Mccarthy Essay Topics Exposed by an Old Pro Finally nice and democratic governments ought to be instituted (Wannebo 97). It means finding different sources of food that might be more abundant. There are those out there. Also, you might wish to have a look at the how-to sites given below. A great deal of writers that are deemed good I consider strange'', he explained. Citations and extracts from assorted sources have to be formatted properly. If you get a compelling reason to find the original, talk to a reference librarian. Both subsequent sentences You aren't going to face the reality. It tells the reader what to anticipate from the remainder of the paper, how you are going to tackle the matter, and directly answers any questions asked of you. This book isn't poetry, however it's likewise not pure narrative. You will not discover any quotation marks within this book. The story ends on an excellent note. The Benefits of the Road Mccarthy Essay Topics Another intriguing moment in this portion of the book is whenever the man claims they must not stay there, although it looks like paradise. There's popular apocalypse, and then there's its antithesis. It's a post-apocalyptic tale told in an extremely minimalist style. Another illustration of symbolism is the use of one gun to signify hope and salvation. Road Mccarthy Essay Topics Ideas Finally, the man dies. He knows that he is sick and knows that he's dying. When he says that he will protect the boy at any cost, it is not an understatement. Another man appears after a number of days. At length, the primary goal of the guy and the boy throughout the full novel is to get to the coast. He decides that his sole purpose in life is to protect the boy from anything that poses a threat to him. It's also telling about the essence of the connection between a guy and a woman and their roles in a family. The guy and the boy fall within this category. The love because of his boy permits him to swear in the male child and starts to realize that the male child is capable of doing the most suitable determinations. Although the male child would like to compose a message in the sand to the fantastic cats the male parent finds it tough to continue being positive. It is hard to erase the image of a youngster and burnt flesh, especially given the simple fact they do not own a father to care for them. In clip the male parent arrives to observe how much his boy has matured and is ready to do the perfect determinations. Unfortunately, the website is too exposed, and they're ma de to move on after a couple of days. All images can be seen at a big size when you're in any reading room at the Library of Congress. No, the product isn't digitized. Yes, it is digitized. Maybe you'll have some luck. My occupation is to look after you. It doesn't really will need to get said that there isn't much hope to be found in a post-apocalyptic setting. The author endeavors to demonstrate the evolution of a human character during the principal protagonists. The Road Mccarthy Essay Topics and the Road Mccarthy Essay Topics - The Perfect Combination The setting is a somewhat depressing scenario in general. The key characters start to starve till they discover another hiding place containing supplies. You should have all pieces of the test under control before you demonstrate your skills in their entire entirety. It won't be any different than every other blood test you've received. What Everybody Dislikes About the Road Mccarthy Essay Topics and Why Ultimately, the book speaks to every one of us. The notion that the days are becoming worse and more morbid than each one prior is extremely clear and it's continuously stressed throughout the book. The guy and the boy, who also stay unnamed throughout the whole novel, travel during the rough terrain of the southeastern United States of america. So, it is going to expand, McCarthy states. the Road Mccarthy Essay Topics: the Ultimate Convenience! The middle of the world is sickened. The author appears to be pointing to the simple fact that with such cruelty, human are likely disappear from the face of the planet. The hope there are others like them that want to rebuild society sustains them. He portrays a lovely soft trout much enjoy the stunning soft world the way it once was before the catastrophe. the Road Mccarthy Essay Topics - Dead or Alive? Mentioning to how it's close impossible to keep up your religion in such difficult times. Folks are in a state of disillusion and it's even tough for one to imagine that things could possibly get much better. By making a place so desolate and hopeless, McCarthy managed to create a need to set up a reason to call home. The undesirable ones appear to take part in acts that demean and even eliminate the ones that are weaker than them.

Race And Ethnicity Cape Verdean American Immigrants Essay

As I read Marilyn Halter’s book, Between Race and Ethnicity: Cape Verdean American Immigrants 1860-1965, I was able to develop a clear perspective of the Cape Verdean’s American voyage as well as their social and economic triumph. Prior to reading this book, I had no knowledge of the Cape Verdean people, unless they are very similar to the â€Å"Brazilians†. Marilyn intentions for her book was to address the social construction of Cape Verdean racial and ethnic identity and how the trials they experience while margining into American society. Cape Verdeans, a mix raced people group of Portuguese and African decent, struggled to attain and maintain their social identity in America, all while enduring isolation and ridicule from both Whites and Black in the new world. After reading this Halters book and her narrative depiction of the Cape Verdeans experience migrating to America. Just as many American immigrants during early 19th and 20th century, they were in sear ch of an opportunity toward social mobility and sustainability while departing from the racial boundaries in their country. Though as soon as they arrived, the American society stressed the Cape Verdeans to choose to identified as white or Black? Little to their knowledge, their journey in search of prosperity immediately immersed them into the vulgar, racial divide between black and white Americans. The Cape Verdeans were a people group that migrated from the Cape Verdes Islands, not too far off the coast West Africa.Show MoreRelatedImportance Of Race Ethnicity : An Exploration Of Asian, Black, Latino, And Multiracial Adolescent Identity Essay1047 Words   |  5 PagesImportance of race-ethnicity: An exploration of Asian, Black, Latino, and Multiracial adolescent identity Article source- Charmaraman, L., Grossman, J. M. (2010, April). Importance of race-ethnicity: An exploration of Asian, Black, Latino, and Multiracial adolescent identity. Cultural Diversity and Ethnic Minority Psychology, 16(2), 144-151. doi: 10.1037/a0018668 (a) Contextual information about the purpose/intention of this study: Throughout the history of United States, race has been one of

Accommodating Employees With Disabilities At The Workplace

ACCOMMODATING EMPLOYEES WITH DISABILITIES IN THE WORKPLACE Presented to Mr. Christopher Lee Chief Executive Officer Fanshawe College Prepared by Dana Alhassan Human Resources Manager MEMORANDUM TO: Dr. Christopher Lee, Chief Executive Officer FROM: Dana Alhassan, Diversity Management Manager SUBJECT: Accommodating Employees with Disabilities in the Workplace DATE: December 1, 2014 As part of Fanshawe College’s diversity initiative, here is the report that I conducted to assess the accommodation of employees with disabilities (EWDs) in our workplace. This study includes a review of employers’ misconceptions about hiring persons with disabilities (PWDs), the benefits of including EWDs in the workplace, and recommended workplace accommodations for EWDs. The results of this study show that although employers often claim to have a diverse workplace, most employers are not very proactive in hiring PWDs and hold beliefs about EWDs that research has proven to be incorrect. Based on the competitive advantage EWDs can offer our institution, we should make a considerable effort to accommodate them. The action plan outlined in this report reflects these advantages and suggests accommodations for EWDs that will benefit our institution and all of its employees and students. It is my hope that this report will provide you with the information required to demonstrate the importance of accommodating EWDs in our institution.Show MoreRelatedCanadian Human Rights Case Study735 Words   |  3 PagesRegulations and Resources Available - Ankeen Relevant Legislation It is crucial for employers and Deaf and Hard-of-Hearing employees to understand and comply with the relevant government legislation in terms of Employment. 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HR departments deal directly with everyone employed by a company. HR can include many ethical pitfalls that can lead to a bad reputation or damage a companies financial sustainability if the situation is notRead MoreEmployee Is Injured On The Job1353 Words   |  6 Pagesregular duties. EKU attempts to return employees to work as quickly as medically possible after an occupational injury. In order to reasonably accommodate restrictions, it is sometimes necessary to assign an employee to a position outside of their regular department. This document describes policies and procedures to be used when an employee is subject to modified or restricted duty by a workplace injury or occupational illness. (All references to â€Å"workplace injury† hereafter also apply to work-related

Expression and Purification of rGFP from E. coli free essay sample

The Expression and Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain, BL21(DE3), Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain, BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained, with elution 3 containing the most amount of fluorescence at approximately 12,000 RFUs. From here, a Bradford assay was performed in order to determine how much protein was in each sample and an SDS-PAGE/Coomassie Blue analysis was done to determine the size and purity of rGFP in the elution 3 sample. The sample came out to be about 34 kDa and was about 75% pure. Lastly, a Western Blot was performed with binding of primary and secondary antibodies to prove that the protein of interest, rGFP, had indeed been expressed and purified. Introduction: Osama Shimomura first discovered Green Fluorescent Protein (GFP) in 1962. The discovery was made inside the bioluminescent jellyfish A. equorea victoria. GFP was found to be a protein of 238 amino acids and had a molecular weight of 26,888 kDa. He was later able to characterize the structure of one of GFP’s unique features- its chromophore, which causes the protein to fluoresce. Later in 1985, Douglas Prasher became the first person to clone GFP (Prasher, 230). For these experiments, a recombinant form of GFP that contained a Histidine6 tag (His6 tag) on the N-terminus of the protein, as well as an X-press epitope tag that begins at the 24th amino acid residue was taken. The His6 tag’s metal ion binding feature was critical during the Ni2+-agarose affinity chromatography because it was able to bind to the nickel inside the column and thus allowed rGFP to be purified since other proteins would be washed out. The purpose of these series of experiments was to allow the expression and purification of rGFP so that further analysis could be done, such as how to determine the purity, size, and total yield of a protein. Finding out this information would give a better understanding of the characteristics of GFP and its significant properties that could lead to further scientific and medical discoveries. Materials and Methods: (Referenced from the â€Å"Biochemistry Lab Manual†) Expression of rGFP in E. coli- Using a single bacterial colony of strain G (BL21pLysS, pRSETA-GFPuv strain from E. coli), 10 ml of LB media [100ug/ml Amp; 25ug/ml Cam] was inoculated and was grown overnight at 37 °C with vigorous shaking until the culture was saturated. Then 500 ml of liquid LB growth media [100ug/ml Amp; 25ug/ml Cam in 1 liter baffled flask, pre-warmed-30 °C] was inoculated with ~4ml of the saturated overnight culture so the culture had an OD600 of 0.1. This culture was grown overnight at 37 °C with vigorous shaking until OD600 of 0.5 was reached. A bacterial pellet designated â€Å"G0† was obtained at time equals zero by pelleting 1ml of culture and discarding the supernatant. The culture was then induced with IPTG (1mM final concentration) and allowed to grow. After 3 hours, a â€Å"G3† bacterial pellet was obtained and both G0 and G3 were stored at -20 °C. Also, 15 ml of the culture was pelleted and the supernatant was discarded, leaving a â€Å"G3-15ml† pellet and was stored at -20 °C to be used later as the starting material to isolate rGFP. Purification of rGFP using Ni2+-Agarose Affinity Chromatography- The rGFP crude extract was prepared before performing the Ni2+-agarose affinity chromatography. The freeze-thaw method was used on the G3-15ml bacterial pellet. The slow freezing at -20 °C causes small ice crystals to form within the bacteria, damaging the bacterial membrane. Addition of 500ul of breaking buffer [10mM Tris, pH 8; 150mM NaCl] twice to the G3-15ml pellet and immediately pipetting the breaking buffer up and down causes the pellet to thaw and the cytoplasm of the bacteria to be released, creating a homogeneous solution. After vortex for 5 minutes, the solution was placed in a 37 °C water bath for 10 minutes. It was then transferred to a rotating platform shaker in a dry air 37 °C incubator for 20 minutes. Once the bacteria had lysed, it was centrifuged at 14,000xg, 4 °C, for 10 minutes. The supernatant was decanted into a new tube and labeled, GCE (rGFP crude extract). The Ni2+-Agarose column was made bypacking in a small amount of glass wool inside a 3 ml syringe. Once secured onto a ring stand with a clamp, a luer-lock was fastened onto the end of the syringe. 2ml of breaking buffer was added to the column and a few drops were excreted before closing the luer-lock to prevent air bubbles. With at ~500ul of breaking buffer in the column, 1ml of 50% Ni2+-agarose slurry was added to the column and the luer-lock was opened to pack the agarose matrix, creating a bed volume of 500ul. The column was then washed with breaking buffer in order to take out any ethanol that the agarose had been stored in. Next, 1ml of GCE was applied and given 5-10 minutes for the his6 tag on the rGFP to bind with the Ni2+-agarose matrix. The luer-lock was then opened to allow ~0.5ml of the effluent to flow into a tube and labeled, W1. The next 0.5ml of the effluent that came out was labeled, W2. Then, breaking buffer was pipetted into the column in 0.5ml increments and each was collected in separate tubes and labeled W3-W10. After the column was rinsed with 5ml of breaking buffer, an elution buffer [10mM Tris, pH 8.0; 150mM NaCl, 300mM imidazole] was added in 0.5ml increments and each of these were collected and labeled E1-310. Determining Protein Concentration of rGFP Fractions- A Bradford assay was used to determine the total protein amount in washes 1-6 and elutions 1-6. First a Bradford standard curve was created using 6 different amounts of Bovine Serum Albumin (BSA)- 0ug, 2.5ug, 5ug, 10ug, 15ug, and 20ug. Each sample was made from a 1mg/ml BSA stock solution and mixed with enough water to bring the volume to a total of 50ul in each tube. 1ml of Bradford reagent was added to each and mixed by vortexing and then incubated at room temperature for 10 minutes. Using a microplate reader, the absorbance at 595nm of each sample could be found. By plotting the data [Absorbance (595nm) vs BSA (ug)], the standard curve was created. The same procedure was done for the W1-6 and E1-6 samples by taking 50ul of each sample and adding 1 ml of the Bradford reagent. Once absorbance was measured, extrapolation of these absorbance values on the standard curve could be done in order to determine the amount of total protein that was present in each sample. SDS-PAGE/Coomassie Blue Analysis- The SDS-PAGE gel was created with 12% resolving gel (used 4X stacking buffer) and 5% stacking gel (used 4X resolving buffer). Both gels contained water, 30% Acrylamide, 10% APS, and TEMED. Using a device made of 2 pieces of glass, the resolving gel was poured in and was solidified before adding the  stacking gel on top. Once it polymerized, a comb was inserted to create the wells in which the samples would be placed. This device was then put inside an electrophoresis tank, which was then filled with electrophoresis buffer. Once the samples (GO, G3, GCE, W3, W4, E3, E4) were prepared with 4X sample loading buffer, they were placed inside the wells and electrophored for 45 minutes at 200 volts. The gel was then removed, stained with Coomassie Blue, and de-stained so that the size and purity of each sample containing the rGFP protein could be determined. SDS-PAGE/Western Blot Development- The SDS-PAGE gel was created, the same prepared samples were loaded, and the electrophoresis was run the same way. This time, the gel was placed inside a cassette (transfer apparatus) with filter paper being placed at the base, then the nitrocellulose (NC) membrane, then the gel, and more filter paper before placing the lid on top. Once the proteins migrated onto the NC membrane, it was stained with 20ml of Ponceau S and de-stained in order to determine the efficiency of the transfer, the orientation of the blot, and to mark the ladder. The blocking step was performed by adding 30ml of 5% non-fat dry milk/TBS solution and incubated for 30 minutes. The membrane was then washed by adding of 30ml of 0.05% Tween 20/TBS and incubated (3X). It was then probed with 7ml of mouse IgG anti-Xpress epitope MAb solution and incubated. The same washing step was repeated 3 times before probing the membrane with 7ml of Sheep IgG anti-mouse IgG conjugated horseradish peroxidase polyclonal anti-serum solution and incubated. After a final wash, 7ml of TMB substrate solution was added, and the membrane was incubated until the desired color intensity was achieved. Results: The expression of rGFP undergoes a process that begins with the induction of IPTG. If no IPTG is induced, the lac repressor will inhibit expression of T7 RNA Polymerase. IPTG inhibits this lac repressor so that a large enough amount of T7 RNA Polymerase is made to overwhelm the inhibition by lysozymes. In figure 1 it is seen that T7 RNA Polymerase allows the T7 promoter in the plasmid to transcribe and translate the rGFP gene. Ampicillin is also to the plasmid to maintain selectivity and protect it from contamination so that only rGFP is being expressed. The T7 promoter will allow the T7 RNA Polymerase to bind to the His6-Xpress-GFPuv tag that’s next to the T7 Promoter and then expression of rGFP will begin. At time equals zero, our G0 sample was collected and then 3 hours after induction with IPTG, our G3 was collected. Both samples were measured qualitatively (handheld UV lamp) and quantitatively (spectrofluorometer). Looking at figure 2, a closer look at the rGFP gene was analyzed. The results showed that the entire gene was 279 amino acids long and contained the His6 tag near the N-terminus, the Xpress epitope tag between the His6 tage and the rGFPuv, and finally the rGFPuv near the C-terminal. Inside the rGFPuv, is the chromophore structure that causes the fluorescence. Since the molecular weight of a single amino acid is 120 Daltons, the MW of this protein can be calculated by multiplying its number of amino acids (279) by the MW of each amino acid (120) giving 33,480 Daltons. A combined activity/elution profile for rGFP was made in order to compare the amount of fluorescence with the total amount of protein in each sample (W1-6, and E1-6) (figure 3). The graph showed the washes to have larger amounts of protein, as was expected since all the undesired proteins were being rinsed out. And although the elutions fewer amounts of protein, they had a greater amount of RFU’s, which indicated being on the right track to knowing whether rGFP had been purified. In the next experiment, an SDS-PAGE gel was created and stained with Coomassie Blue (figure 4). Between elutions 2 and 3, the third one looked the most prominent and therefore was assumed to be the rGFP band. It was located around the 34 kDa marker on the ladder, which is fairly close to the calculated MW of the rGFP protein. Other bands smaller than this were also visible, but at this time could not be identified as a contaminant or rGFP with C-terminus degradation. The band looked to be about 75% pure. In the final experiment, a Western Blot was developed in order to determine whether the proteins had transferred onto the NC membrane and if it was indeed the rGFP protein (figure 5). Undesired proteins were blocked with 5% non-fat dry milk/TBS solution, and then the proteins left were probed with the primary antibody. This primary antibody should have bound to the Xpress-epitope tag in rGFP and when probed with the secondary antibody, it  should have bound on several spots of the primary in order to amplify the signal. After washed with TBS substrate solution, the Western Blot proved that rGFP had been successfully purified and that it was the band at 34 kDa. The Western Blot also showed that the smaller bands were merely contaminants since they did not show up in the final product. Conclusion/Discussion: The attempt to express and purify rGFP from these series of experiments was successful. The Ni2+-Agarose affinity chromatography purified the protein by allowing other proteins to be washed out and rGFP to be collected in the elution samples. The qualitative (handheld UV lamp) and quantitative (spectrofluorometer) measurements taken showed some fluorescence in the washes and this was probably due to the N-terminus (containing the His6 tag) being degraded and therefore not being able to bind to the column. The third elution (E3) ended up having the most amount of rGFP and this was proven with the Bradford assay. The SDS-PAGE/Coomassie Blue Analysis experiment then helped to identify the purity and size of the rGFP in this third elution. With the purity being approximately 75% and the band showing up around the 34 kDa marker, the conclusion was made that rGFP was indeed purified by the Ni2+-Agarose column and that the calculated molecular weight was similar to what was shown on the gel. However, there were some bands at a lower molecular weight that did show up on the gel, but at this time it could not be determined whether they were contaminants or rGFP that had had their C-terminus degraded. In order to determine that the band found in E3 was in fact rGFP, the SDS-PAGE process was done again and this time stained with Ponceau S and a Western Blot was performed. The primary antibody was able to bind to rGFP’s unique feature, the Xpress-epitope tag, and then the secondary antibody amplified and conjugated it. Because the antibodies were able to bind and the visible markings showed up in the Western Blot, this was able to prove that the bands on the gel were indeed rGFP. The fact that they didn’t bind to the lower bands with the smaller molecular weight brought on the conclusion that there were simply contaminants that did not contain the Xpress-epitope tag. After these conclusions were made, follow-up experiments could be performed to see how useful GFP could be. One experiment that could be done is taking the gene that encodes this green fluorescent protein and transfecting the cells of another organism to see if it could produce fluorescence as well. Another experiment could be taking GFP and using it as a marker to tag a protein of interest to see this protein’s activity and its’ localization in the cell (Chalfie, et. al.). Experiments with GFP could prove to be very helpful in the world of science by discovering the expression and activity of proteins and how they can affect living organisms. References: Rippel, Scott. Biochemistry Lab Manual. Print. Prasher, Douglas C., Virginia K. Echenrode, William W. Ward, Frank G. Prendergast, and Milton J. Cormier. â€Å"Primary Structure of the Aequorea Victoria Green-Fluorescent Protein.† Gene 111 (1992): 229-233 Chalfie, Martin. â€Å"Green Fluorescent Protein as a Marker for Gene Expression.† National Center for Biotechnology Information. U.S. National Library of Medicine. Web. 04 Aug. 2013. http://www.ncbi.nlm.nih.gov/pubmed/8303295. Figure 5. Western Blot of rGFP Fractions. Figure Captions Figure 1. Plasmid Map of rGFP from E. coli strain, BL21(DE3). IPTG inhibits the lac repressor protein allowing T7 RNA polymerase to be produced. The PT7 promotes expression on His6-Xpress-GFPuv and placed in a media that contain ampicillin to maintain selectivity so that only rGFP is produced Figure 2. The schematic diagram of rGFP from the E. coli strain, BL21(DE3). The numbers indicate where each amino acid sequence begins. The Histidine-6 tag begins at the 5th amino acid residue. The Xpress Epitope tag begins at the 24th amino acid residue. Finally, the rGFPuv sequence, being 238 residues long, begins at the 39th amino acid residue. It also contains the chromophore (65-67 residues of rGFPuv, Ser-Gly-Tyr) which is located from the 103rd-105th residue. This diagram displays the entire sequebce of the rGFP protein, coming out to be 279 amino acids long. Figure 3. Combined activity/elution Profile of rGFP during washes 1-6 and elutions 1-6 in a Ni2+-agarose column. The graph shows RFU’s (left) and total protein in ug (right) for each wash and elution. Once the rGFP had been purified in the Ni2+-agarose column, a breaking buffer (10mM Tris, pH 8.0; 150mM NaCl) was used to create the washes by pipetting the buffer in 0.5ml increments and collecting each in separate tubes labeling them W1-W6. Then, an elution buffer (10mM Tris, pH 8.0; 150mM NaCl; 300mM imidazole) was collected in increments of 0.5ml and labeled E1-E6. We could measure the amount of RFU’s each sample had using 200ul of each fraction and placing them in a spectrofluorometer. E3 showed to have the greatest amount of fluorescence. Using the Bradford assay, the total protein of each sample was determined by extrapolating points from the standard BSA curve. Both values were scaled up. Figure 4. SDS-PAGE gel/Coomassie Blue of rGFP fractions from E. coli strain, BL21(DE3). The gel was created by using 12% resolving gel which was composed of water, 4X resolving buffer (0.75M Tris, pH 8.8, 0.4% SDS), 30% Acrylamide (29.2% w/v acrylamide, 0.8% w/v bis acrylamide), 10% APS (ammonium persulfate), and TEMED (tetramethylenediamineAsds). Once solidified, a 5% stacking gel composed of water, 4X stacking buffer (0.25M Tris, pH 6.8, 0.4% SDS), the same 30% Acrylamide, 10% APS, and TEMED was added. Once the stacking gel was polymerized, the gel was ready to be loaded with fractions of the E. coli strain, BL21(DE3), that we collected from previous lab. A molecular weight marker (ladder) was also loaded onto the gel. Going left to right, lanes 1and 2 are disregarded, the G3, G0, GCE, W1, W2, E2, E3, ladder. The gel was placed in a gel electrophoresis tank and filled with electrophoresis buffer (450ml H2O, 50ml 10X stock solution (30g Tris, 144g glycine, 10g SDSD per liter). Figure 5. Western blot of the rGFP samples. An SDS-PAGE gel was created with 12% resolving gel and 5% stacking gel and ran through the electrophoresis procedure. The gel was then placed in a cassette and on top of a nitrocellulose membrane so that the protein could transfer from the gel to the membrane. The Ponceau S staining identified the presence of rGFP. It was then blocked with 5% non-fat dry milk/TBS solution and probed with a primary antibody, mouse IgG anti-Xpress epitope MAb solution. It binds uniquely to the Xpress-epitope tag in rGFP so it can be isolate. It was then probed with the second antibody, Sheep IgG anti-mouse IgG conjugated horse radish peroxidase polyclonal anti-serum solution. This would amplify and conjugate the rGFP and finally, it was washed with TMB substrate solution to create the color intensity desired.

School Ethos free essay sample

This display reflects the diversity in our school by allowing the children to create self images they are able to understand different ethnicities and backgrounds and relate to them. [pic]ample Evaluate how effective this method of communicating the school ethos, mission, aims and values is. We will write a custom essay sample on School Ethos or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page You may wish to compare it with other methods the school uses. You may wish to compare it with how other schools communicate their ethos, mission, aims and values. Culturally inclusive schools reflect and celebrate the cultural, religious and linguistic diversity in the school, the community. Diversity is reflected in wall displays and the achievements of all learners are celebrated. Simon marks is a welcoming place for students and parents from different backgrounds, the makeup of the staff at all levels and the governing body reflects diversity within the community. Explain how this reflects school ethos, mission, aims and values. This displays reflects Simon mark aim to allow the children to express their creative abilities through art and even music, due to our tremendous music department [pic] Evaluate how effective this method of communicating the school ethos, mission, aims and values is. You may wish to compare it with other methods the school uses. You may wish to compare it with how other schools communicate their ethos, mission, aims and values. Parents will think about their childs personality, learning style, and any special needs. Does the child need the structure that a traditional school setting would provide, or does he or she prefer to explore and take more personal responsibility for learning, Could she benefit from some type of alternative schooling approach, Does the child respond differently to being in small and large groups, If for example a child learns best in small cooperative work groups, then parents may want to consider finding out if Simon marks uses this instructional strategy. If a child has a special interest in music or art, Simon marks can explain this through displays such as this. This in turn etherising Simon marks mission statement and school ethos,

Argumentative Essay on Banning Tobacco Is It Effective

Argumentative Essay on Banning Tobacco Is It Effective Free sample argumentative essay on Banning Tobacco: There are many ways to stop people from using drugs such as tobacco instead of prohibition there are infinite possibilities that you can do to stop people from drinking or smoking. Whether we like it or not we can not stop everyone from drinking or smoking and that’s the same as not being able to prevent what happens to innocent civilians like how a drunk driver can hit your child. Or you can’t stop the effects of drugs like tobacco which influence you in a really bad way which leads to poor judgments. So you really have no control over someone else’s life not to do drugs. Well, there are ways to help prevent it but it’s really not anyone’s choice if you can drink or smoke. One way to help prevent people from wanting to take tobacco is to make laws that prohibit if you abuse the ability to take drugs too much. Well I think there is a law like that but if you make it worse like making a longer prison period or a larger payment fee it may work. Some people think there is nothing left for them in this life or they got nothing to lose if they do drugs so we should help those people out and try to force help upon them even though we can’t. I really think no matter what we do people will continue to smoke or take drugs because no matter what happens people still took drugs we banned many illegal drugs on the street. Even if there is a law or chance to get arrested people take that chance and risk it all for a couple minutes of pleasure people think drugs are the answer if all else didn’t help so that is why they turn to drugs. So no matter what law we come up with or anything else we try to do to stop drugs there is no guarantee all of it will go away so no matter what drugs are here to stay. So have hope, maybe you can save one or two lives but not every ones. So one person can make all the difference for you so drugs are going to always be a problem no matter what anyone’s opinion is.

Infection among Burn Patients Research Paper Example | Topics and Well Written Essays - 2000 words

Infection among Burn Patients - Research Paper Example In general 75% of the fatalities are usually seen at the scene of the incident itself, and for those reaching medical care, infection is the major cause of mortality and illness (Murray, 2011). Since majority of the skin is exposed to the elements and to the atmosphere, protecting it from infection and bacteria is a major challenge. For those with burn injuries, this challenge is even bigger. The type and quantity of microorganisms which colonize the burn wounds are also known to affect the patient’s future risk for would infection. The pathogens which infect the wound are mostly gram-positive bacteria which include methicillin-resistant Staphylococcus aureus (MRSA) and gram-negative bacteria Acinetobacter baumannii-calcoaceticus complex, Pseudomonas aeruginosa and Klebsiella species (Murray, 2011). These infective agents often increase the resistance of a person to various antimicrobials. Moreover, burns are usually infected with fungus infective agents, making the infection process on burn wounds even more difficult to prevent and manage. In recent years, the survival rates and the infection rates for infection among burn patients have improved with the introduction of modern medical practices. These medical practices now include advances in fluid resuscitation, nutritional support, pulmonary care, burn wound care, and infection control (Church, et.al., 2006). Despite these improvements, there are still improvements which can be made to the practice. (Murray, 2011).  

Analyses the processes of innovation implemented during new venture Essay

Analyses the processes of innovation implemented during new venture creation through the study of a Small to Medium Enterprise ( - Essay Example Thus, it is important to analyse the processes involved in the creation of a venture and this can be clearly illustrated through the study of SMEs. Especially in the case of understanding innovation for a new venture, SMEs are perfect models in order to understand the entire process involved. This essay tries to discuss the processes of innovation involved in the creation of new venture through understanding SMEs. SME and its environment The SMEs are affected by the internal and business environment because they are all integral parts of the existing innovative process of the global business. As of the present trend in conducting business, SMEs are the basic units of a country’s affairs in commerce and trade. Thus, there is a need to analyse the recent business environment that substantially influenced SMEs significant moves. PEST Analysis Politics, economy, social and technological aspects are essential parts of the business environment. All of these components are affecting SMEs at certain level. After all, SMEs are organisations or companies with specific structure, operation and resources that are highly influenced by politics, economy, social and technological factors. ... This is the current scenario especially among SMEs in which their opportunities lie on the ability of the government to integrate its political power into the business environment. Thus, SMEs are trying to create important efforts to eliminate whatever existing gaps that may arise along the process. Economy The recent global economic recession is said to be worst economic downturn in history as next to Great Depression (Marino et al., 2010). It is in this reason that companies are making extra effort in order to recover from corresponding profit lose they incurred from such economic event. In line with this, SMEs are under important moves in order to ensure continuous business operation. Social The business environment without question is composed of individuals who are in their daily activities may be influenced by the information they received from the environment. One of this may be due to the effort of companies to stimulate needs for their offerings. Kotler (1999) discussed that companies are into formulating strategies that eventually will stimulate needs for their offerings. This in return may set the current social trend in which the business sectors have gained considerable opportunity for their growth. In line with this, SMEs are challenged to formulate their own strategy in order to also gain social acceptance. Technology The transfer of knowledge and technology is politically desired by the government but this is hindered by issues such as problems related with communication and technology down to intellectual property regimes (Dhillon et al., 2009). The real issue therefore is on how SMEs can eventually adapt into technological competency as the latest trend in the business environment. Aspects of

The life of Garibaldi Essay Example for Free

The life of Garibaldi Essay Get the clamp ready and place the tile on the clamp, then place the conical flask on the tile. Rinse your burette with your diluted HCl to replace any previous liquids which may intervene in the results. Now secure the burette to the clamp making sure the nozzle is facing in the conical flask. You should now have your apparatus set-up like this: 30/5230/35230/image006. gif Funnel Burette filled with diluted HCl Conical Flask Clamp Tile Ca(OH) and Indicator i Now everything should be ready. From your volumetric flask, empty out the diluted HCl into a normal beaker. Using this beaker fill the burette up to the 0 mark with a funnel. i Start titrating slowly until the solution has totally reacted. Then record the results noting how much HCl you used. Record the results in a suitable format and repeat the experiment at least three more time for more accuracy. Finally record the average (mean) titre. i SAFTEY: this is extremely important as you are handling hydrochloric acid. Goggles must be worn at all times, hair must be tied back and bags must remain under the desk. If anything goes wrong, consult the teacher immediately. Always remember to work safely as HCl can be hazardous to your skin, the indicator may stain your clothing as well. (Accuracy): The pipette, burette and volumetric flask only have a percentage error of + or 0. 1%. This is a small error. That is why they are used in this experiment. Be sure to take precautions such as making sure that when you pour in the HCl or Ca(OH) you do not let it run down the sides because some will be left on the sides thus making an inaccuracy. As you can see above, the bold says10. 0cm3 of your Ca(OH), when instead it should have been 25. 0cm3. The reason for this is because there was not enough of the Ca(OH) to perform all the experiments. This is a limitation due to the fact that 25. 0cm3 would have been a more accurate measure because 10. 0cm3 may not be enough for a clear result. AnalysisJ Rough Accurate 1 Accurate 2 Accurate 3 Final burette reading cm3 37. 3 37. 2 37. 1 37. 3 Initial burette reading cm3 0 0 0 0 Volume used cm3 37. 3 37. 2 37. 1 37. 3 Mean titre cm3 37. 3 37. 2 37. 1 37. 3 This is the final part to the investigation. In this part of the investigation I aim to determine the final concentration of the limewater solution. I will go through my calculation step by step and explain what I am doing along the way. Starting off, lets look at what we have. The equation for the reaction is: 30/5230/35230/image001. gif Ca(OH)2(aq) + 2HCl(aq) CaCl(aq) + 2H2O(L). For the Ca(OH) we are trying to find the concentration in mol g dm-3. However we have the volume (10. 0cm3 is how much we er solution. This would change the colour of the solution to light orange which would change to pink by the end of the titration. 5. After that I will place the flask onto a white tile placed on a clamp stand. Then I will open the tap of the burette that is held in the clamp stand and let the acid neutralise the alkali. 6. By the end of titration the limewater solution turns pink which indicates that it has been neutralised. 7. I will repeat the experiment at least thrice to make it a fair test. Repeating the experiment will also ensure that the results that I get are accurate. The reaction that takes place can be described with the help of this balanced equation: Ca (OH)2 + 2HCl i CaCl2 + 2H2O In order to get reliable results from the exper at the end point would be reached before the equivalence. Variable Control You need t ensure that you take your chemicals from the same batch so as not to get two different concentrations each time you test them. Temperature and pressure should have no effect on the reaction Organisation Set up the equipment as shown in the diagram below, ensuring that the burette valve is closed. Run de-ionised water through the burette and wash all of the equipment with it First of all dilute the hydrochloric acid using by adding slowly 25ml of 2.00moldm-3 HCl to 475ml of de-ionised water in the 500ml beaker. Then, using a pipette, put 100ml of the HCl solution into the burette Put 50ml of limewater into a conical flask using the pipette then add 5 drops of methyl orange to the solution, this should turn the solution a pale orange Open the .. . molarity 1dm3 = 10x10x10cm3 = 1000cm3 1(Molarmass) = 8. 12gdm3 Evaluation My practical experiment went quite well and I got a variety of results, which were suitable for the task given. My results show that I got two identical results and one just 1. 1cm3 off. Which suggests that I have been accurate and precise during the experiment. By being accurate it came about by specific apparatus I used, which restricted errors. However during the experiment I came across an array o

Whitman and Neruda as Grassroots Poets Essay -- Poet Poetry Poem Paper

Whitman and Neruda as Grassroots Poets â€Å"The familial bond between the two poets [Walt Whitman and Pablo Neruda] points not only to a much-needed reckoning of the affinity between the two hemispheres, but to a deeper need to establish a basis for an American identity: ‘roots,’ as Neruda referred to his fundamental link with Whitman† (Nolan 33). Both Walt Whitman and Pablo Neruda have been referred to as poets of the people, although it is argued that Neruda with his city and country house, his extensive travels, and his political connections, was never really â€Å"one† of the mass. Nonetheless, his work and energies went into supporting the common working man, and not the elite. By the late 1940’s Neruda had openly defined himself as a communist, looking for the equal treatment of all citizens of Peru. Whitman, though not overtly political like Neruda, did emphasize the equality between all in his writing. The appellation, â€Å"poet of the people,† is used to indicate their sympathies towards a commonality in humans, if not the â€Å"common man†. As the term â€Å"commoner† carries various connotations and needs much explaining, I prefer to discuss the two authors as grassroots poets. â€Å"Poets of the people† and â€Å"grassroots poets† have many similarities, but b y using the term grassroots I draw on grassroots theater studies which illuminate certain artistic purposes and themes. Thinking of Whitman and Neruda as grassroots poets can deepen our understanding of their personas and their work, and especially indicate a similarity of purpose between the two poets who employed different structural styles of writing. First and foremost, the term â€Å"grassroots† hinges on a sense of community. It implies a political motivation from the bo... ...nity theatre is to create a dialectic between the present state and future possibilities of particular communities, moderated by a knowledge of, and an identification with, those communities† (Kershaw, 61). With this basic understanding of â€Å"grassroots† with in the context of community theater, let us proceed to a comparative study of grassroots sentiments in excerpts from Neruda’s The Heights of Macchu Picchu, and Walt Whitman’s Song of Myself. Go to analysis Works Cited: Kershaw, Baz. The Politics of Performance. Radical Theatre as Cultural Intervention. New York: Routledge, 1992. Nolan, James. Poet-Chief. The Native American Poetics of Walt Whitman and Pablo Neruda. Albuquerque: University of New Mexico Press, 1994. Whitman, Walt. Leaves of Grass (1855). in Walt Whitman Poetry and Prose. New York: The Library of America, 1996.

Revolutionary Mothers Essay

Carol Berkin masterfully presents a glimpse of the lives of the women who were affected by the Revolutionary War through many different eyes, views, and opinions in Revolutionary Mothers: Women in the Struggle for Independence. She paints a vivid picture in your mind of how the war affected these women by not focusing on one race or political view, but rather through multiple races and political views. Throughout this book, the author describes the women of the Revolution as courageous, yet intelligent, strong, yet resourceful. In Berkin’s writings, her admiration for these women is apparent. She describes how these women felt as if they new that they had very important roles in the war, however they didn’t see them as much more than supporting the men who actually were meant to run the country. As strong willed as many of these women were, they made weak attempts at equal rights during these male dominated times. This book was given validity through supporting texts. Berkin used other authors such as Elizabeth Ellet to drive her points home. She is able to give even more personal insight from letter correspondences as well as diaries from women who were caught up in the midst of these times. Finally, Berkin uses documents such as the Philipsburg Proclamation, the Edenton Resolves, and the Book of Negroes as a foundation to much of the support or neglect thereof for the Americans. I believe that the author did an outstanding job of presenting the information in this book. Many authors would have tried to intertwine these women’s stories in order to make the story more interesting as a whole. Instead she sets aside a chapter to different groups she wants to go into detail discussing. For instance, she doesn’t try to compare and contrast the women who were on the home front to the women who followed the armies. Instead, she helps you realize the importance of both by devoting time to each group. This allows us to learn more about these women and get a grasp of what they really went through. The multiple number of resources Berkin used keep this book interesting. She does a good job of making sure there are notes in the back to go along with each chapter in the case that you want to, or need to, learn more about the chapter or to receive expanded content. Also, these notes give you a great resource for continuing your research on that chapters topic. I consider myself somewhat of a history buff. I had learned much about the wars and causes of the Revolutionary War. I had seen many documentaries about the war. This book however lent it’s hand to a completely different side of the war. You never learn about the women who are left at home, nor the women who follow the armies, nor the women who were acting as spies. My knowledge has been impacted greatly by this book. My eyes have been opened to a whole new side of the war that you never even knew existed. Overall I believe this book was a great, and relevant book for this course. I think it was interesting as a whole because of the extensive resources Berkin used. I will admit that there were some dry parts that I had to work my way through, but the knowledge gained was worth the fighting through.

245 - 835 Words

WIRRAL AUTISTIC SOCIETY evidence CENTRE NUMBER 770001 number Observation/assessor Observation/expert witness Written account Tick Tick Tick Witness testimony Knowledge questions Professional discussion Tick Tick Tick CANDIDATE †¦Louise Farquhar†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ DATE †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦ ASSESSOR †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦. †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦. UNIT †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦... Unit Outcome Assessment details 4222-245 Outcome 5.1†¦show more content†¦This may reflect a false belief that a disabled boss will be less effective than a non-disabled boss. In this day and age its wrong the so many are fighting to get their voice heard and although attitudes are changing and have considerably over the past 3 years there is still a great unbalance for equality The coalition promised at a Mental Health Strategy that they would work to empower both the individual with a disability and the employers to move forward and that its wouldn’t just be a social marketing paper excise , hopefully the new government will also carry this forward in real terms Many employers have seen the benefits of employing individuals with a disability as they are sometimes more productive Education has also come along way and there is more choice and opportunities for an individual to study to a high level Give examples ofShow MoreRelatedHcs 245 Culture and Disease Paper990 Words   |  4 PagesCulture and Disease Paper - Malaria Erin E. 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